產(chǎn)品詳情
簡(jiǎn)單介紹：
干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白與其它公司提供的重組蛋白不同，rHuMIG/CXCL9蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白
| Synonyms | Monokine-like Protein |
| Species | Human |
| Accession | Q07325 |
| GeneID | 4283 |
| Source | Escherichia coli. |
| Molecular Weight | 干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 103 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADV KELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMIG/CXCL9 as determined by LAL method. |
| Reconstitution | 干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 干擾素-γ/ CXCL9誘導(dǎo)的重組人單核因子蛋白 | |
| SDS-PAGE |
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| Reference |
1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22. |
| Background | CXCL9 is a T-cell chemoattractant induced by IFN-γ belonging to the CXC chemokine family and it is also known as Monokine induced by gamma interferon (MIG). CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11 and they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant human CXCL9 contains 103 amino acids which is a single non-glycosylated polypeptide chain. The human CXCL9 shares 75 % and 67 % a.a. sequence identity with mouse and rat CXCL9. |
